Studying Flow Mediated Responses in Isolated Vasculature – April 28, 2016
|Dr. Éric Thorin, Ph.D.
Professor, Université de Montréal
The physiological significance and effects of flow on controlling and coordinating vascular function are well-appreciated. However, flow-mediated regulation of vascular function is a complex and difficult mechanism to study experimentally. Care must be taken to select appropriate instrumentation to allow for precise control of intravascular pressure and intraluminal flow, while minimizing artifacts introduced by the small size of glass cannulae. With proper simultaneous control of intravascular pressure and intraluminal flow, the researcher will be able to explore such responses as flow-mediated dilation, flow-induced constriction and other physiological responses that depend upon shear stress in the vasculature.
During this webinar Dr. Éric Thorin, a leading expert in the effects of shear stress in the vasculature, explains key concepts in setting up a system for the purpose of examining flow-mediated responses in isolated blood vessels. The webinar sponsor, Living Systems Instrumentation, has been supplying tools for in vitro studies of cardiovascular function for over 20 years. Viewers will gain an understanding of how to setup and utilize a pressure arteriograph capable of simultaneous control of intravascular pressure and intraluminal flow.
- Required equipment
- How to setup and calibrate instrumentation
- Best practices, including how to minimize cannulae resistance
- Sample experimental protocols and applications
About Our Presenter:
Dr. Éric Thorin has a long-standing interest in the study and mechanisms of ageing related to the vascular endothelium. His laboratory has developed several approaches to investigate the functional consequences of ageing combined with risk factors for cardiovascular diseases (CVD) on the evolution of a reversible endothelial dysfunction to an irreversible vascular disease. His main areas of research include the study of the cerebrovascular and peripheral vascular dysfunction in the mouse model of human dyslipidemia and atherosclerosis, the molecular mechanisms leading to endothelial cell senescence and the impact of risk factors for CVD in patients with obesity, diabetes and coronary artery disease.