Quick Summary
 Single & Dual Vessel Chamber Models
For Perfusion of Isolated, Cannulated Vessels

     These chambers are machined from an inert plastic (PET) that has chemical and physical properties superior to acrylics. The chambers can be readily mounted on the microscope mechanical stage for adjustment of the image field.

Single Vessel Chambers- Most can be supplied with Pt stimulation electrodes for electrical neurotransmitter release from the nerve varicosities. Add /S to model number for this option. All of these chambers are ideal for applications requiring confocal microscopy and simultaneous fluorescence and diameter measurements.  For ratiometric fluorescence and vessel dimensioning data acquisition systems, consider IonOptix www.ionoptix.com.

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 Model  Description

CH/1/AU

Sealed chamber for applying extravascular pressures to a perfused, pressurized vessel and for long-term perfusion experiments.  Bath volume: 3-5 ml. Requires superfusion for warming bath.
CH/1/AU/SH

Similar to CH/1/AU, but includes Self-Heated bath and temperature controller.  May be used with or without superfusion.

CH/1

For fluorescent, confocal, and many other applications. Requires superfusion for warming bath.
CH/1/SH

Same as CH/1 but includes Self-Heated bath and temperature controller. May be used with or without superfusion.
CH/1/QT

For rapid freezing or fixation of cannulated, pressurized vessel
CH/1/R

For axially rotating or inverting a cannulated, pressurized vessel.
 

Dual Vessel Chambers - Separate baths, 3-8 ml. Removable covers for control of gas environment above each bath. Holder provided for pH electrode (Model PH/E1) for measurement in either bath. Add /S to model number to add Pt stimulation electrode option for electrical neurotransmitter release from the nerve varicosities.

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 Model  Description
CH/2/B

Requires superfusion for warming bath solution.

CH/2/B/SH

Self Heated version of CH/2/B.  A supplied heat controller (TC-03) sets and regulates both bath temperatures thereby eliminating the need for superfusion with buffers such as HEPES. Superfusion is needed to warm bath with other buffers.